The keys to reach success when insemination dogs are:

  • Inseminating the right time.
  • Ensuring an adequate number of viable sperm per dose.
  • Good preparation and handling of semen.
  • A good deposition of semen in the female genital tract.
  • Timing of insemination

Achieving success in insemination depends largely on the deposition of semen at the time of ovulation. Keep in mind that the dog is a female monoestrus with 1 or 2 estrous per year. Another interesting feature is that the ovulated  oocyte is immature and requires 96-108 hours in the uterine horns to mature and develop into an egg capable of being fertilized by sperm. Therefore, the approach of the time of insemination or natural service will be a key step to success.


Determination of progesterone will be our invaluable guide that will tell us objectively what stage of ovulation is our dog. However, their detection is expensive and requires frequent punctures, so we will rely on various smears without great cost or effort that indicate how it is transforming the vaginal mucosa. Other detection methods are the detction of follicles by vaginal ultrasonography or endoscopy.


These vaginal determinations should be performed at intervals of 2-3 days.

The epithelial cells of the vagina become as hormonal response to ovarian follicular development. So when we visualize small round cells is that our dog is not estrogenic phase. When we see large cell, straight and angular edges, no small nucleus and cytoplasm is that they have cornified and therefore there are oestrogenic (estrus is starting).

At the moment we have> 70% cornified cells, we must begin to evaluate progesterone in blood plasma. At the beginning of estrus progesterone should be around 2 to 3 ng / ml, indicating that ovulation will occur within 2 days. The day of ovulation (day 0 called “zero”), progesterone concentration was determined between 4 and 10 ng / ml. The increase in rounded cells and neutrophils tells us that we have begun the next stage ovarian, diestrus.

Heat cycle phases

  • Proestrus (follicular phase). Vaginal smears with blood cells. Duration + -15 days.
  • Estro (female accepts the male). Duration of 8-15 days. Time of ovulation in the end.
  • Metaestrus. 21 days.
  • Right. 3 months.

Timing of insemination

With cooled semen, proceed to insemination the day of ovulation and a second dose 2 days after.

With frozen and thawed semen, whereas oocytes need 2 days to maturity, the insemination is performed 2 days after the time of ovulation, and the second dose 48 hours later. However, practice and individual knowledge of the species, an experience which will grant you adjust these parameters to achieve more precise tolerance in timing the insemination.