The amount of semen that is required to give the best chance of a successful insemination varies depending on the size of the female.

• For bitches less than 10 pounds (5 kg) 1.5 to 3 mls of semen are required per insemination.
• For bitches 10 to 50 pounds (5 to 25 kg), 3 to 5 mls of semen is necessary.
• For bitches over 50 pounds (25 kg), 5 to 8 mls of semen is necessary.

After collection of the semen, a semen extender, MRA Can, can be added to nourish the semen and to achieve the necessary amount of semen. For samples to be used within 24 hours but not immediately, an extender fluid is added to protect and nourish the sperm. This sample is then chilled and preferrably be used within 24 hours. For samples intended for freezing, a different type of extended is added to protect the sperm from the effects of freezing.