There are many factors that can affect the final quality of frozen semen. The age of the male at the time of the seminal freezing is one of the most important so it is recommended to choose males between 2 and 6 years, based primarily on animal genetic and reproductive potential.
The general health of the male is also very important, the presence of temperature peaks, vaccinations or stress situations might cause poor semen quality at the time of collection.
Many males require the presence of a female in heat to ejaculate and some studies suggest that the presence of a female in heat will increase the quality of seminal ejaculation by boosting the male libido.
We should note that a male that has never been used for breeding or that has not been used for a long period of time will require several pickups, at least two weeks, before freezing.